In a methane digester where fermentation can be stopped normally, the rate of methanogens producing methane depends on the rate of application of non-methanogens to organic synthesis. Shandong Biogas Purification Equipment Company reminds us that in order to further improve the production of biogas, it is not the continued enrichment of methanogenic bacteria, but the hydrolysis rate of non-methanogenic bacteria to organic matters, especially the synthesis rate of cellulose. Therefore, it is an important measure to improve the application rate of organic matter synthesis and biogas production to provide dominant microbial flora for biogas digesters and improve the metabolic function of non-methanogenic bacteria.

为了进步沼气池发酵原料的合成应用率和产气量，国内外都非常注重在沼气池内坚持较多的产甲烷细菌群(厌氧污泥)。除了应采取上述措施外，为沼气池提供水解细菌的优势菌群，改善非产甲烷细菌的代谢功用，使有机物得到更彻底的合成，是进步沼气池产气量的重要途径。关于处置固形物含量较高的、含较多较难合成的有机物的沼气发酵原料，采取这一措施显得尤为重要。

In order to improve the synthetic application rate and gas production of biogas digester fermentation raw materials, more methanogenic bacteria (anaerobic sludge) are attached great importance to in biogas digesters at home and abroad. In addition to the above measures, it is an important way to improve the gas production of biogas digesters by providing the dominant bacterial flora of hydrolytic bacteria, improving the metabolic function of non-methanogenic bacteria and making organic matter more thoroughly synthesized. It is particularly important to take this measure to dispose of biogas fermentation raw materials with high solid content and more difficult to synthesize organic matter.

山东沼气提纯设备公司研讨发现，在猪粪和鸡粪的厌氧消化过程中，各种水解酶(蛋白酶、纤维素酶、脂肪酶和淀粉酶等)的酶活与沼气产量成正比关系，水解酶活均呈现在产气峰值的左近，并且水解酶活与沼气产量的变化曲线类似，这一结果在国内外尚未见报道。另有研讨标明，适量添加水解酶能加快发酵原料中纤维素的降解速度，明显进步沼气产量。

Shandong Biogas Purification Equipment Co., Ltd. has found that in the anaerobic digestion process of pig manure and chicken manure, the activities of various hydrolytic enzymes (protease, cellulase, lipase and amylase, etc.) are proportional to the biogas production, and the activities of hydrolytic enzymes are near the peak value of biogas production, and the change curve of hydrolytic enzymes is similar to that of biogas production. This result has not been reported at home and abroad. It was also indicated that proper addition of hydrolytic enzymes could accelerate the degradation of cellulose in fermentation raw materials and significantly improve biogas production.

自然状态下的秸秆由纤维素、半纤维素、木质素及局部蛋白质、氨基酸、单宁等组成。由于自然木质纤维素构造的复杂性，停止生物转化的难点在于如何高效将它降解成为可发酵糖。分离预处置办法消弭木质素的障碍作用，是进步纤维素被纤维素酶水解的有效步骤。将纤维素酶的产生、纤维素的水解及酒精发酵过程有效组合，可进步生物转化效率降低转化本钱。大家晓得，麦秸、稻草、玉米秆等植物性原料表皮上都有一层蜡质，假如不作处置就下池，水分不易经过蜡质层进入秸秆内部，纤维素很难腐朽合成，不能被甲烷细菌应用，而且会形成浮料或结壳现象。

The natural straw is composed of cellulose, hemicellulose, lignin, local protein, amino acid, tannin and so on. Because of the complexity of natural lignocellulose structure, the difficulty of stopping bioconversion is how to efficiently degrade it into fermented sugar. Separation pretreatment is an effective step to improve the hydrolysis of cellulose by cellulase. The combination of cellulase production, cellulose hydrolysis and alcohol fermentation can improve the efficiency of bioconversion and reduce the cost of conversion. It is well known that there is a layer of wax on the epidermis of plant materials such as wheat straw, rice straw and corn straw. If it is not disposed of, it will go down into the pond. Water will not easily enter the straw through the waxy layer. Cellulose is difficult to decay and synthesize, and can not be used by methane bacteria, and it will form bubbles or crusts.

由于微生物的木质纤维素降解才能较弱，招致农作物秸秆的水解过程迟缓，水解水平低，使农作物秸秆的厌氧消化时间长、消化率低、产气量少、投入产出效益差，因此限制了秸秆大范围地应用于厌氧发酵。

Because the degradation ability of lignocellulose by microorganisms is weak, the hydrolysis process of crop straw is slow and the level of hydrolysis is low, the anaerobic digestion time of crop straw is long, the digestibility is low, the gas production is low, and the input-output benefit is poor, so the wide application of straw in anaerobic fermentation is limited.

因而，研讨如何经过预处置使作物秸秆中的木质纤维素得到有效降解成为秸秆生物处置的一个关键。

Therefore, how to effectively degrade lignocellulose in crop straw after pretreatment has become a key issue in straw biological disposal.

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